Figure 5: Effect of 6-aryl-9-substituted-6,9-diazaspiro-[4,5]decane-8,10- diones series 1– 9 on percentage swelling in carrageenan-induced paw edema. Mice were injected with compounds 1– 9 (25 mg/kg, intraperitoneal) and diclofenac sodium (D) (10 mg/kg, intraperitoneal) or vehicle (C) 1 h before carrageenan challenge. The mouse hind paw was injected with 0.05 ml of 0.5% suspension of carrageenan in saline into the plantar tissue. The other hind paw was injected with an equal volume of saline and served as control. The thickness of the mouse hind paws was measured with a Vernier Caliper 1, 2, and 3 h after carrageenan challenge. The percentage swelling was calculated by measuring the difference between the thicknesses of the two paws. The data represent the mean percentage swelling ± SEM. *Significant difference from control at P < 0.05; #Significant difference from diclofenac sodium at P < 0.05; @significant difference from compounds 1, 2, and 3 at P < 0 .05.