Users Online: 30
Home
Current Issue
Ahead of Print
Search
About Us
Editorial Board
Archives
Submit Article
Instructions
Subscribe
Contacts
Reader Login
Export selected to
Endnote
Reference Manager
Procite
Medlars Format
RefWorks Format
BibTex Format
Citation statistics : Table of Contents
2015| January-April | Volume 14 | Issue 1
Online since
April 8, 2015
Archives
Previous Issue
Next Issue
Most popular articles
Most cited articles
Show all abstracts
Show selected abstracts
Export selected to
Cited
Viewed
PDF
ORIGINAL ARTICLES
Production and prebiotic activity of exopolysaccharides derived from some probiotics
Magdel-Din M Hussein, Mohamed F Ghaly, Mona Y Osman, Al Shimaa G Shalaby, Mohamed MI Helal
January-April 2015, 14(1):1-9
DOI
:10.4103/1687-4315.154687
Objective
The aim of this study was to focus on exopolysaccharides (EPSs) produced by
Lactobacillus delbrueckii bulgaricus
,
Lactobacillus helveticus
or
Lactobacillus casei
and their use as a prebiotic for
Lactobacillus reuteri
,
Lactobacillus rhamnosus
,
Lactobacillus acidophilus
or
Bifidobacterium bifidum
.
Materials and methods
Optimization of culture conditions using different carbon and nitrogen sources and different temperature, pH and incubation periods for maximum EPS production was studied.
Results and conclusion
It was found that the best conditions were as follows: the use of sucrose (20%) instead of glucose in MRS medium, incubation at pH 7.0 and temperature 37°C for 72 h incubation under anaerobic conditions to give the highest EPS yield; a yield of 13.99 g/l was recorded in case of
L. helveticus
when grown on the aforementioned optimized conditions. It was found that
L. delbrueckii bulgaricus
EPS has the highest prebiotic indices (I), varying from 7.9 to 10.1. In contrast,
L. helveticus
and
L. casei
EPSs have the lowest prebiotic indices (I), varying from 1.4 to 2.4.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
11
5,348
734
Levofloxacin: formulation and
in-vitro
evaluation of alginate and chitosan nanospheres
Ramadoss Arun Balaji, Sathya Raghunathan, Radhakrishnan Revathy
January-April 2015, 14(1):30-35
DOI
:10.4103/1687-4315.154705
Background and objectives
Levofloxacin, the active l-isomer of ofloxacin, is a widely used fluoroquinolone, with activity against bacteria that causes respiratory, skin, and genitourinary tract infections, (-)-(S)-9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7H-pyrido benzoxazine-6-carboxylic acid hemihydrates. It is a new quinolone antimicrobial agent that exhibits broad-spectrum
in-vitro
bactericidal activities against gram-positive and gram-negative aerobes. The aim of this study was to formulate sodium alginate nanospheres containing levofloxacin and evaluate its physiochemical properties, exploring alternative routes of administration, such as nanoparticle to develop a targeted drug delivery system and to act locally on the organ of infection with enriched therapeutic efficacy.
Materials and methods
Sodium alginate and calcium chloride solutions were prepared. A constant volume (20 μl) of levofloxacin solution was incorporated into the sodium alginate solution, and then the same method was followed for the preparation of hybrid chitosan-alginate nanoparticles.
In-vitro
release study was carried out by dialysis membrane for 7 h in the physiological fluid (pH 7.4 phosphate buffer solution). Morphology and structure characterization of nanoparticles were investigated by field emission scanning electron microscope and Fourier transform infrared spectra, zeta potential, X-ray diffraction, particle size analysis, respectively.
Results and conclusion
This paper reports the possibility to entrap lipophilic levofloxacin within chitosan/alginate (CS/ALG) nanoparticles using a very simple ionotropic pregelation technique; strong electrostatic interactions exist in the nanoparticles. The nanoparticles with a diameter of 25-55 nm were obtained at the optimal mass range of sodium alginate: calcium chloride:chitosan in the meta acid environment. The delivery behavior of levofloxacin from nanoparticles was studied. Levofloxacin released from chitosan-alginate nanoparticles was 71% at pH 7.4 within 7 h. The release profile was characterized by an initial burst effect in phosphate buffer solution, followed by a continuous and controlled release phase. The drug release mechanism from polymer also offers an interesting potential for the delivery of lipophilic compound.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
11
5,550
776
Estimation of total phenolic, tannins, and flavonoid contents and antioxidant activity of
Cedrus deodara
heart wood extracts
Sourabh Jain, Aakanchha Jain, Sanjay Jain, Neelesh Malviya, Vikas Jain, Dharmendar Kumar
January-April 2015, 14(1):10-14
DOI
:10.4103/1687-4315.154690
Purpose
The present study was investigated to determine
in-vitro
antioxidant activity and total phenolic, total flavonoids, and total tannins contents of extracts of
Cedrus deodara
heart wood.
Materials and methods
Antioxidant activity of aqueous and alcoholic extract of
C. deodara
heart wood was evaluated against 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picryl hydrazyl (DPPH), and hydroxyl radical-scavenging activity models. Total flavonoids, tannins, and phenolic content of
C. deodara
were also determined.
Results and conclusion
Among both extracts, aqueous extract showed the highest total phenolic contents (23.97 μg/g of gallic acid equivalent/g of extract). In DPPH, superoxide anion, and ABTS scavenging test, the IC
50
(μg/ml) value of aqueous and alcoholic extract was 61.89, 75.79, 87.76, 121.55, 115.29, and 122.42, respectively. Hence, the above evidences suggest that
C. deodara
heart wood is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
8
7,728
847
Isolation and microbiological identification of bacterial contaminants in food and household surfaces: How to deal safely
Amal S Othman
January-April 2015, 14(1):50-55
DOI
:10.4103/1687-4315.154720
Objective
This study investigates and reveals the relationship between pathogenic bacteria in some types of food and that present in different household sites (kitchens) and determines an effective disinfecting method to eliminate bacteria from common kitchen locations, some of which could be harmful or pathogenic.
Materials and methods
A total of 90 samples were collected; 85 samples were taken from different sites from five home kitchens and five samples were collected from different types of food. Samples were obtained (before and after disinfection) from kitchen towels, cooking gas stove knobs, refrigerator handles, water taps, and kitchen sponges used for washing utensils by using sterile cotton swabs. Bacteria were identified according to the conventional biochemical methods. DNA fragmentation was done to show the effect of disinfectants on the most common bacteria.
Results and conclusion
Escherichia coli
,
Klebsiella
spp., and
Staphylococcus aureus
were the most abundant bacteria in the isolates. After disinfection using disinfectants containing sodium perborate and sodium silicate (detergent), sodium hypochlorite (Clorox), 5% amphoteric surfactant and chlorine (dishwashing powder), and Dettol, the samples were free of bacterial contamination. There was also a correlation between food contamination and bacteria isolated from the kitchens. As
E. coli
was the most highly abundant pathogen in the kitchen and was removed by the tested disinfectants, it was chosen for DNA fragmentation assay to examine the effect of the disinfectants on the bacterial DNA. Kitchen towels, cooking gas stove knobs, refrigerator handles, water taps, and kitchen sponges are the most common sites in kitchens that transmit pathogenic bacteria. They must be disinfected routinely after preparing food.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
5
40,530
2,223
Synthesis, cytotoxic, proapoptotic evaluation, and molecular docking study of some new
N
-substituted sulfonyl-3-indolyl heterocycles
Eslam R El-Sawy, Heba M Abo-Salem, Shaymaa M Yahya, Manal S Ebaid, Adel H Mandour
January-April 2015, 14(1):15-29
DOI
:10.4103/1687-4315.154695
Background and objectives
Apoptosis, also called programmed cell death, is a fundamental biological phenomenon that plays a crucial role in processes such as immune regulation, embryogenesis, and general tissue homeostasis. B-cell lymphocyte/leukemia-2 (
BCL
-2) family members are key regulators of apoptosis. The ability of the indole derivative to disrupt microtubule assembly and induce G2/M arrest, polyploidy, and apoptosis through mitochondrial pathways in COLO 205 cell has been reported; in addition, it reduced the levels of procaspase-3, procaspase-9,
BCL
-xL, and
BCL
-2 gene. The aim of this study is to describe the synthesis of some new
N
-substituted sulfonyl-3-indolyl heterocycles and to study their cytotoxic and proapoptotic effects. In addition, a molecular docking study of the most biologically active compounds against the
BCL
-2 protein is discussed.
Materials and methods
A new series of triazolopyridines
3a-c
, diaminopyridines
4a-c
, acetamides
5a-c
, triazolo[1,5-
a
]pyridines
6a-c-8a-c
, pyrido[1,2-
b
][1,2,4]triazines
9a-c
,
10a-c
, pyrazoles
11a-c, 12a-c
, and pyrimidine derivatives
13a-c-15a-c
were prepared by an initial reaction of 2-((
N
-substituted sulfonyl-1
H
-indol-3-yl)methylene) malononitriles
2a-c
with different reagents. The newly synthesized compounds were tested for their cytotoxic activity against the HepG2 cell line. The compounds that showed promising IC
50
values were chosen for the study of their proapoptotic effect on the
BCL
-2 gene, which is an antiapoptotic factor, and they significantly inhibited the expression levels of the
BCL
-2 gene. The binding mode of the most promising proapoptotic compounds was assessed by docking studies with the CHIMAERIC
BCL
2-XL protein (PDB ID: 2W3L).
Results and conclusion
From the data obtained, the most active compounds against the HepG2 cancer cell line were in the descending order of
10b>4c>10a>10c
, whereas compounds
3c
,
6c
,
9c
,
4b
, and 5c showed moderate to slight growth inhibition. Compounds
4c
,
5c
,
9c
,
10a
,
10b
, and
10c
significantly inhibited the expression levels of the
BCL
-2 gene. Docking results showed that compounds
5c
and
9c
showed good fitting within the pocket site of the protein molecular surface and had a minimum binding energy of −20.29 and −18.98 kJ/mol, respectively, in comparison with the co-crystallized ligand, which is in agreement with the experimental result of a proapoptotic effect.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
4
2,713
368
Phytoconstituents from
Curcuma longa
L. aqueous ethanol extract and its immunomodulatory effect on diabetic infected rats
Manal H Shabana, Eman M Shahy, Mona M Taha, Gehan M Mahdy, Maha H Mahmoud
January-April 2015, 14(1):36-43
DOI
:10.4103/1687-4315.154713
Background and objectives
Curcuma longa
L. (
C. longa
) has been shown to possess a wide variety of pharmacological properties against malignant proliferation, inflammation, and parasitosis. Further,
C. longa
has been reported to possess antioxidant and hypoglycemic activities. The aim of this study was to isolate and elucidate the structure of the phytoconstituents of turmeric and test the hypoglycemic, antioxidant, antibacterial, and anti-inflammatory properties of the alcohol extract of
C. longa
on diabetic rats infected with
Staphylococcus aureus
.
Materials and methods
The extraction and isolation of the constituents of
C. longa
were performed according to the standard methods of column, paper, and thin-layer chromatography. The chemical structure of the isolated compounds was established by R
f
, by characterization of acid hydrolysates, by ultraviolet spectral analysis, and by
1
H-NMR spectroscopy. Serum glucose, immunoglobulin E (IgE), nitric oxide, cytokine levels (IL-6, TNF-α, and IL-1β), and blood cell counts (total and differential) were determined.
Results and conclusion
Three compounds were isolated from the alcohol extract of
C. longa
. These compounds were identified as apigenin 7-
O
-rhamnoside 4′-
O
-glucoside, a new compound isolated for the first time from nature, and 7-methoxyapigenin-6-
C-
glucoside and
N
-(3-methoxyphenyl) acetamide, two known compounds isolated from
C. longa
for the first time. Also, the alcohol extract of
C. longa
ameliorated the serum glucose, IgE, nitric oxide, and cytokine levels in diabetic infected rats with
S. aureus
.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
4
4,252
384
The composition of the lipoidal matter of the seeds of
Pleiogynium timorense
(DC.) Leenh
Ataa A Said, Elsayed A Aboutabl, Ahmed A Hussein, Gehan F Abdel Raoof
January-April 2015, 14(1):65-68
DOI
:10.4103/1687-4315.154725
Background and objectives
Nothing dealing with the chemistry of the seeds of
Pleiogynium timorense
(DC.) Leenh. could be traced in the available literature. The objective of the present work was to investigate the lipoidal matter from
P. timorense
seeds and to isolate and identify the major sterols and triterpenes in the petroleum ether extract.
Materials and methods
The petroleum ether extract of
P. timorense
seeds was prepared. The extract was subjected to column chromatography using silica gel (type 60-230 mesh) as an adsorbent. The unsaponifiable matter and fatty acid methyl esters were analyzed by GC/MS.
Results and conclusion
GC/MS analysis of the unsaponifiable matter from the petroleum ether extract of
P. timorense
seeds revealed the identification of 70.04% of unoxygenated compounds and 23.34% oxygenated compounds. Hydroxylated compounds, ketones, and steroidal compounds represent 23.24, 0.20, and 3.19%, respectively. 1-Heptene (66.47%) was the major compound in the USM, followed by butylated hydroxy toluene (21.07%). GC/MS analysis of fatty acid methyl esters showed that nine fatty acid methyl ester derivatives could be identified, representing 95.84% of the total composition. The major fatty acid was 9,12-octadecadienoic acid (linoleic acid) (33.8%). Saturated fatty acids represent 36.93% of the total fatty acid content, whereas monounsaturated and diunsaturated fatty acids represent 25.11 and 33.8% of the total fatty acid content, respectively. Column chromatography of the petroleum ether extract led to the isolation of two compounds [α-amyrin and 5,24(28)-cholestadien-24-methylen-3β-ol].
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
2
2,111
180
Preliminary clinical trials of karela,
Momordica charantia
, on non-insulin-dependent diabetes mellitus patients
Mouchira Abdel Salam, Souad E El-Gengaihi, Emad N Zikry
January-April 2015, 14(1):69-74
DOI
:10.4103/1687-4315.154728
Background
Traditional methods of treatment for diabetes was recommended by WHO in 1985. On
in-vivo
trials in animal models, one of the authors revealed the potentiality of karela in the treatment of diabetes.
Aim
The aim of the study was to evaluate the effects of
Momordica charantia
as a hypoglycemic in type 2 diabetic patients. Formulated tablets from
M
.
charantia
(karela) juice of the unripe fruits were used.
Materials and methods
Juice from immature fruits was filtrated through specific membranes to separate active polypeptide mimic insulin. The powder obtained after spray drying was formulated as a 20 mg tablet of polypeptide. Eighteen of 60 patients continued the treatment. All patients had type 2 diabetes. They were followed up for 1-8 weeks and examined clinically and investigated for different parameters.
Results
As this trial is a case analysis, the result of each case was presented and the overall trial results were discussed.
Conclusion
M. charantia
can be used as an effective oral adjunct hypoglycemic, with no reportable clinical side effects. The treatment must be continued for 4 weeks.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
1
4,803
429
Formulation and evaluation of fast-dissolving films of lisinopril
Prabhakara Prabhu, Akhilesh Dubey, Karthik Kamath
January-April 2015, 14(1):56-64
DOI
:10.4103/1687-4315.154723
Objective
The aim of this study was to formulate and evaluate the oral fast-dissolving film of lisinopril for the effective management of hypertension and cardiac diseases.
Materials and methods
Fast-dissolving films were prepared by the solvent-casting method using a combination of different polymers, HPMC E5 LV, HPMC E 3 and HPMC 4KM, along with PEG as a plasticizer. The Fourier-transform infrared study for the drug-polymer interaction was carried out. Evaluation of physical parameters such as physical appearance, surface texture, uniformity of weight, uniformity of strip thickness, surface pH, folding endurance, uniformity of drug content and percentage of moisture absorption were performed. Kinetic data analysis for the release study and the stability study were also performed.
Result and conclusion
Results of uniformity of weight, thickness, folding endurance, surface pH, tensile strength, percentage drug content, swelling index, tensile strength and percentage elongation of all the films were found to be satisfactory with respect to variation of these parameters between films of same formulation. The Fourier-transform infrared study indicated that there was no interaction between the drug and the polymers. The
in-vitro
drug release study showed that a better rate of drug release was achieved by formulations FA3, FB1, FB4 FC8 and FD10 compared with other formulations. The stability study did not show any significant difference in the external appearance, the drug content and the
in-vitro
drug release. The
ex-vivo
study indicated that the drug has a better ability to cross the sublingual barrier at a faster rate, and hence the delivery system was found to be promising as it has the potential of overcoming the drawbacks associated with tablet formulations available in the market presently.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
1
13,067
729
Evaluation of bronchodilatory properties of fruits of
Opuntia elatior
Mill
Sanjay P Chauhan, Navin R Sheth, BN Suhagia
January-April 2015, 14(1):44-49
DOI
:10.4103/1687-4315.154717
Background
Traditionally, fruits of
Opuntia elatior
Mill. were used for their immunomodulatory and antiasthmatic action. The presence of potentially active nutrients and their multifunctional properties make prickly pear a perfect candidate for the production of phytopharmaceutical products.
Objective
The objective of the present study was to evaluate the bronchodilatory properties of fruits of
O. elatior
Mill.
Materials and methods
The bronchodilatory properties were evaluated using bronchospasm induced by acetylcholine and histamine, anticholinergic action on isolated rat ileum, and antihistaminic action on isolated guinea pig ileum.
Result and discussion
Bronchodilating effect of fruit juice was dose dependent against spasm induced by acetylcholine and histamine.
O. elatior
Mill. fruits possess a significant inhibitory effect on rat and guinea pig ileum contraction through antihistaminic and antimuscarinic action.
Conclusion
This study suggested that fruits of
O. elatior
Mill. possess a significant inhibitory effect on rat and guinea pig ileum, and betacyanin, an active principle compound in prickly pear, may be responsible for the action.
[ABSTRACT]
[FULL TEXT]
[PDF]
[Mobile Full text]
[EPub]
[CITATIONS]
1
2,499
192
Feedback
Sitemap
|
What's New
|
Feedback
|
Disclaimer
|
Privacy Notice
© Egyptian Pharmaceutical Journal | Published by Wolters Kluwer -
Medknow
Online since 31st Dec, 2013