ORIGINAL ARTICLE
Year : 2020  |  Volume : 19  |  Issue : 4  |  Page : 321-329

Optimization and molecular characterization of novel Aspergillus spp. producing invertase enzyme


Department of Microbial Genetics, National Research Centre, Dokki, Giza, Egypt

Correspondence Address:
PhD Rasha G Salim
Department of Microbial Genetics, National Research Centre, Dokki, Giza, 12622
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/epj.epj_34_20

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Background and objective Invertase, one of the most important enzymes used in industrial and pharmaceutical applications, has been produced from different Aspergillus spp. Statistical and mathematical tools [response surface methodology (RSM)] have been carried out to optimize culture conditions to facilitate maximum enzyme production. This investigation aims to screen the ability of some Aspergillus spp. to produce invertase enzyme, to optimize the culture parameters, and to detect the genetic diversity between them. Materials and methods A total of five fungal strains were tested for the production of invertase. The most promising strain was selected for optimization. Optimization experiments were carried out to obtain maximum enzyme production, and a molecular marker (RAPD-PCR) was used to differentiate between these strains. Results and conclusion Some Aspergillus spp. were examined to produced invertase enzyme after 2 days of cultivation. Aspergillus niger recorded the highest enzyme production (6.8 U/ml). Ten RAPD-PCR primers were used to differentiate between Aspergillus spp. A total of 131 bands were amplified in the five strains of Aspergillus spp. Based on RAPD-PCR analysis, the highest similarities obtained between A. parasiticus and A. niger showed 75%. Moreover, the RAPD-PCR technique is a good tool for differentiation between the Aspergillus spp. To optimize culture condition, RSM was used to increase invertase production by A. niger. Temperature, pH, and incubation time dramatically affect invertase yield. A combination of these factors (pH 5, 35°C, and incubation for 72 h) was optimum for maximum production of invertase (8.2 U/ml). Our results employed RSM to elucidate the combination of effective factors such as temperature, pH, and incubation time to obtain a high yield of an important enzyme, invertase, from A. niger, used as a promising candidate for industrial applications.


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