Year : 2020  |  Volume : 19  |  Issue : 3  |  Page : 238-243

Production and one-step purification of serratiopeptidase enzyme from Serratia marcescens with potent anti-inflammatory and antioxidant power

1 Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Giza, Egypt
2 Department of Plant Biochemistry, National Research Centre, Dokki, Giza, Egypt

Correspondence Address:
Mostafa A El-Abd
Department of Chemistry of Natural and Microbial Products, Lab 174, National Research Centre, Dokki 12311, Giza
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/epj.epj_65_19

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Background and objective Inflammation is a huge problem facing the world. NSAIDs are the most popular and widespread drugs used for lowering the impaired symptoms accompanied with inflammation. This group has plentiful disorders to the gastrointestinal tract reaching to gastric ulcer. The objective of this study was to produce a natural product having an anti-inflammatory power without the side effects concomitant with the used drugs. The serratiopeptidase enzyme has a potent anti-inflammatory action without any abnormal disorders. Materials and methods Different media and cultural conditions were evaluated for the optimal production of the serratiopeptidase enzyme from a local bacterial strain. Partial purification was employed using the ammonium sulfate precipitation technique. Results and conclusion Serratiopeptidase enzyme was produced by the bacterial strain Serratia marcescens and its anti-inflammatory and antioxidant power were declared. The best medium was chosen for the optimal productivity and the optimum pH was 6 that recorded the highest anti-inflammatory and anti-oxidant activity at 67.5 and 71.8%, respectively, which is powerful than any chemical drugs used. Serratiopeptidase enzyme produced by this strain was partially purified by ammonium sulfate precipitation revealed an increase in specific activity by two times than the crude one with 14.7 recovery. The authors conclude the use of serratiopeptidase enzyme for treating the inflammation instead of the traditional ways.

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