| ORIGINAL ARTICLE |
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| Year : 2020 | Volume
: 19
| Issue : 2 | Page : 188-196 |
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Evaluation of the antivirulence activity of ethyl acetate extract of Deverra tortuosa (Desf) against Candida albicans
Mohamed H Sharaf
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt
Correspondence Address:
PhD in Medical Microbiology Mohamed H Sharaf
Alshohadaa Street, Nasr City, Cairo, 11884
Egypt
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/epj.epj_10_20
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Objective This study aimed to assess the efficiency of ethyl acetate (EtOAc) extract of Deverra tortuosa (Desf) against Candida albicans.
Materials and methods The antifungal activity of the EtOAc extract of D. tortuosa was evaluated using the paper disk diffusion method. The minimum inhibitory concentrations were assessed using broth dilution methods. The antivirulence efficiency of the EtOAc extract was assessed through the evaluation of antibiofilm using the broth dilution method, reduction in fungal dimorphism using spider and GLcNAc media, and assessment of phospholipase activity using egg yolk emulsion medium. The time-kill assay of the EtOAc extract was assessed. Cytotoxicity evaluation of the EtOAc extract against normal cell line MRC-5 using MTT assay was done. The compounds in extract were analyzed using gas chromatography-mass spectrometry.
Results and conclusion The inhibition zone of the EtOAc extract of D. tortuosa was 26 mm and minimum inhibitory concentrations was 12.5 mg/ml against C. albicans. The antibiofilm activity of the EtOAc extract showed inhibition of up to 52.2% at a concentration of 25 mg/ml. The EtOAc extract showed a complete reduction in fungal dimorphism and transition between yeast cell and hyphae at concentration of 25 mg/ml. The time-kill assay showed inhibition activity at different concentrations in a dose-dependent manner with −2.6 log10 CFU after 24 h at 25 mg/ml. Our results support the in vitro potential of D. tortuosa extract as anti-C. albicans. Gas chromatography-mass spectrometry indicated that 23 peaks were observed. Five (74.53%) antimicrobial compounds were present in considerable amounts, including 9-octadecenoic acid (Z)-, methyl ester/hexadecanoic acid, methyl ester/9,12-octadecadienoic acid (Z, Z)-, methyl ester/octadecanoic acid, methyl ester/phenol,2,4-bis (1,1-dimethyl ethyl)-, and another 18 compounds comprised 25.47%. Cytotoxic activity of EtOAc against MRC-5 cells showed little toxicity, with IC50 exceeding 249 µg/ml after 24 h of incubation.
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