| ORIGINAL ARTICLE |
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| Year : 2019 | Volume
: 18
| Issue : 1 | Page : 27-41 |
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Biochemical studies and biological activities on L-glutaminase from rhizosphere soil Streptomyces rochei SAH2_CWMSG
Hassan M Awad1, Azza M.Noor El-Deen1, El-Sayed E Mostafa2, Amany A Hassabo2
1 Chemistry of Natural and Microbial Products, Department Pharmaceutical and Drug Industries Research Division, National Research Centre, Giza, Egypt
2 Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt
Correspondence Address:
Hassan M Awad
Department of Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Research Division, National Research Centre, PO Box: 12622, Dokki, Giza
Egypt
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/epj.epj_32_18
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Background and objective L-glutaminase (L-GLUNase) is a potential anticancer enzyme that hydrolyzes amide bond of L-glutamine to give glutamate and ammonium ion. It is used as an antioxidant, a flavor enhancing agent and a biosensor for glutamine level measurement. The aim was to produce L-GLUNase in high yield from a promising local Streptomyces isolate for many pharmaceutical applications.
Materials and methods A total of 20 Streptomyces isolates for their capacity of L-GLUNase production were screened. A potent L-GLUNase producer, SAH2_CWMSG isolate, was identified by phenotypic and phylogenetic analysis. L-GLUNase was purified using ammonium sulfate followed by gel filtration on Sephadex G-100. The purified L-GLUNase was characterized, and its application as an antimicrobial, anticancer, and antioxidant agent was investigated.
Results and conclusion The phylogenetic analysis of SAH2_CWMSG strain confirmed that the SAH2_CWMSG strain was most similar to Streptomyces rochei (99%). It produced L-GLUNase activity of 58 U/ml under shake flask submerged fermentation. The purified L-GLUNase has the molecular weight of 55 kDa and Km and Vmax value of 1.314 mmol/l and 95.24 μ Me/min, respectively. Of the various physiochemical parameters tested, pH 7.5 and temperature 40°C were optimal for the enzyme activity. On the contrary, 10 mmol/l of Mn+2 showed a slight increase in L-GLUNase activity. A promising Streptomyces sp. fully identified as S. rochei SAH2_CWMSG (Gen Bank ID: KU720627) is an efficient source of L-GLUNase production. Therefore, it can be potentially used as enzyme supplement, which has many industrial and pharmaceutical applications. |
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