Year : 2013  |  Volume : 12  |  Issue : 1  |  Page : 40-45

Biotransformation of soybean saponin to soyasapogenol B by Aspergillus parasiticus

1 Department of Chemistry of Natural and Microbial Products, National Research Center, Dokki, Egypt
2 Department of Microbiology, Faculty of Science, Ain shams University, Cairo, Egypt

Correspondence Address:
Hala A. Amin
PhD, Department of Chemistry of Natural and Microbial Products, National Research Center, Dokki, 12311 Cairo
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Source of Support: None, Conflict of Interest: None

DOI: 10.7123/01.EPJ.0000427332.31862.10

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The aim of this study was to select of the most potent fungus that is able to hydrolyze soybean saponin (SS) to soyasapogenol B (SB). The selected fungus was cultivated under different physiological conditions to evaluate its ability to transform SS to achieve the maximal conversion output.

Materials and methods

Within 72 h, the biotransformation of SS by Aspergillus parasiticus, followed by isolation and purification of SB as a main product were carried out. The identity of SB was established by determination of its RF value and IR, mass spectra, and NMR spectra. Furthermore, different sets of experiments were carried out to enhance the activity of the tested organism and consequently, SB production.

Results and conclusion

Screening of different fungal isolates for transformation of SS to SB revealed that A. parasiticus produced the highest yield of SB. The maximum SB yield was obtained using a production medium composed of (%, w/v): malt extract, 4; yeast extract, 2; KH2PO4, 0.2; (NH4)2SO4, 0.2; MgSO4·7H2O, 0.03; CaCl2·2H2O, 0.03; galactose, 0.5; and SS, 3 (pH 8). The medium was inoculated with 6% (v/v) inoculum of a 72 h old culture and incubated on a rotary shaker (150 rpm) at 30πC for 72 h. Under these optimal conditions, the cell biotransformation efficiency was increased from 13.44 to 65%.

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