Year : 2012  |  Volume : 11  |  Issue : 2  |  Page : 93-98

Characterization and purification of the crude Trematosphaeria mangrovei laccase enzyme

1 Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, Egypt
2 Department of Botany, Faculty of Girls for Arts, Science and Education, Ain Shams University, Cairo, Egypt

Correspondence Address:
Eman R. Hamed
PhD, Department of Chemistry of Natural and Microbial Products, National Research Centre, 12311 Dokki, Cairo
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.7123/01.EPJ.0000419801.40087.2a

Rights and Permissions


The aim of this work was to study the purification and characterization of the crude extracellular laccase produced by the marine-derived fungus Trematosphaeria mangrovei.


The general properties of the crude laccase enzyme produced by T. mangrovei were investigated. These include the effect of temperature, pH, thermal and pH stabilities, and enzyme and substrate concentrations on the laccase activity. Partial purification of the T. mangrovei laccase enzyme was carried out by fractional precipitation with ammonium sulphate, ethanol and acetone. Further purification was carried out on a Sephadex G-100 column.

Results and conclusion

The results obtained showed that the crude enzyme reached its maximal activity at 35πC, pH 4.5, at an enzyme concentration of 5.429 mg protein/reaction mixture and at a substrate concentration of 40 mmol/l 2,2-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid). The enzyme was stable for 60 min at 35πC and retained about 80–90% of its activity after treatment for 60 min from 40 to 50πC. The enzyme showed maximum stability (100%) at pH 4.5 and 91.6% at pH 4.0 after 60 min. Fractional precipitation of the fungal extracellular T. mangrovei laccase enzyme with different methods showed that the enzyme fraction precipitated at 60% acetone was the most favourable enzyme fraction; it showed 4.84 purification fold. Laccase obtained from the 50–60% acetone fraction was purified by Sephadex G-100. The final preparation thus obtained reached 31.47-fold that of the culture filtrate (1466.49 U/mg protein) and showed a single band on native polyacrylamide gel electrophoresis.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded210    
    Comments [Add]    

Recommend this journal