|Year : 2015 | Volume
| Issue : 1 | Page : 69-74
Preliminary clinical trials of karela, Momordica charantia, on non-insulin-dependent diabetes mellitus patients
Mouchira Abdel Salam1, Souad E El-Gengaihi Ph.D 2, Emad N Zikry1
1 Department of Complementary Medicine, National Research Centre, Dokki, Cairo, Egypt
2 Medicinal and Aromatic Plants Department, National Research Centre, Dokki, Cairo, Egypt
|Date of Submission||24-Dec-2014|
|Date of Acceptance||09-Feb-2015|
|Date of Web Publication||8-Apr-2015|
Souad E El-Gengaihi
Medicinal and Aromatic Plants Department, National Research Centre, 12622 Dokki, Cairo
Source of Support: None, Conflict of Interest: None
Traditional methods of treatment for diabetes was recommended by WHO in 1985. On in-vivo trials in animal models, one of the authors revealed the potentiality of karela in the treatment of diabetes.
The aim of the study was to evaluate the effects of Momordica charantia as a hypoglycemic in type 2 diabetic patients. Formulated tablets from M. charantia (karela) juice of the unripe fruits were used.
Materials and methods
Juice from immature fruits was filtrated through specific membranes to separate active polypeptide mimic insulin. The powder obtained after spray drying was formulated as a 20 mg tablet of polypeptide. Eighteen of 60 patients continued the treatment. All patients had type 2 diabetes. They were followed up for 1-8 weeks and examined clinically and investigated for different parameters.
As this trial is a case analysis, the result of each case was presented and the overall trial results were discussed.
M. charantia can be used as an effective oral adjunct hypoglycemic, with no reportable clinical side effects. The treatment must be continued for 4 weeks.
Keywords: Hypoglycemic, karela tablets, type 2 diabetes
|How to cite this article:|
Salam MA, El-Gengaihi SE, Zikry EN. Preliminary clinical trials of karela, Momordica charantia, on non-insulin-dependent diabetes mellitus patients. Egypt Pharmaceut J 2015;14:69-74
|How to cite this URL:|
Salam MA, El-Gengaihi SE, Zikry EN. Preliminary clinical trials of karela, Momordica charantia, on non-insulin-dependent diabetes mellitus patients. Egypt Pharmaceut J [serial online] 2015 [cited 2020 Oct 30];14:69-74. Available from: http://www.epj.eg.net/text.asp?2015/14/1/69/154728
| Introduction|| |
The earliest recorded treatment for diabetes mellitus involved the use of plants. The papyrus Ebres of 1550 BC recommended a high fiber diet of wheat grains and okra. A multitude of herbs, spices, and other plant materials have been described for the treatment of diabetes worldwide (Baily and Day; 1988) . Since the availability of insulin, folklore medicines for diabetes have almost disappeared in occidental societies, although they continue to be the cornerstone of therapy in underdeveloped countries.
Renewed attention to alternative medicines and natural therapies has stimulated a new wave of research interest in traditional practices, and the WHO expert committee on diabetes has listed it as one of its recommendations that traditional methods of treatment for diabetes should be further investigated .
Baily and Day 1989 demonstrated that more than 400 traditional plant treatments for diabetes mellitus have been recorded, but only a small number of them have received scientific and medical evaluation to assess their efficacy. A hypoglycemic action from some treatments has been confirmed in animal models and non-insulin-dependent diabetic patients. Various hypoglycemic compounds have been identified.
Baily et al.  stated that botanical substitute for insulin seems unlikely, but traditional treatment methods may provide valuable clues for the development of new oral hypoglycemic agent and simple dietary adjuncts.
Momordica charantia (MC), bitter melon, karela, balsam pear, the plant involved in this investigation, is a remedy used mainly in West Africa and exerts a mild hypoglycemic effect in healthy rabbits . In India and China, the herb has been crushed and dried to form tablets ,.
Consumption of 50 ml of aqueous extract of karela with a 50 g of oral glucose challenge reduced glucose concentrations of non-insulin-dependent diabetes mellitus by 20% within 1 h . Many studies conducted on MC using animal models [7-10] proved that oral consumption of karela does not enhance insulin release, although an aqueous extract has been shown to stimulate insulin release from normal isolated islets in vitro. It has also been reported that acute administration of karela juice with glucose load resulted in a significant improvement in glucose tolerance without increasing insulin levels in blood. In a study carried out in nine Asian non-insulin-dependent diabetes mellitus patients living in the UK, a significant reduction in glycosylated hemoglobin was recorded, indicating an improved control of blood glucose levels after 8-11 weeks .
It was reported that treatment of diabetic patients with powdered fruit for 3-7 weeks led to a mean fall of 25% (range 11-48%) in postprandial (PP) glucose levels. There was a marked fall in both blood and urine sugar after 7 weeks in a group treated with an aqueous extract of the fruit. Glycosylated hemoglobin showed significant reduction by the end of the trial .
Karela juice shows certain insulin mimetic effects such as increased glucose uptake into muscle, stimulation of lipogenesis, and inhibition of lipolysis on tissue preparation in vitro .
Therefore, the aim of this study was to clinically evaluate the hypoglycemic effect of the tablet formulated from a polypeptide of karela fruits.
| Materials and methods|| |
Immature fruits of karela were squeezed to a juice form. The juice obtained was filtered after centrifugation. The filtrate was again filtered through membranes of regenerated cellulose with a molecular weight cutoff of 30 000 and 10 000 kg Da successively. The obtained juice was evaporated under reduced pressure (40°C) to a syrupy-like matter. The syrup obtained was spray dried after mixing with multidextrene in a special ratio. The powder obtained was analyzed to estimate the polypeptide in it. This powder was formulated as a tablet having a polypeptide of 20 mg.
| Patients|| |
Eighteen patients could be followed up from the Internal Medicine Clinic of the Medical Service Unit of the NRC. All patients had type 2 diabetes. They were examined clinically and investigated for fasting blood sugar (FBS), 2 h PP blood sugar, glycosylated hemoglobin, liver function tests, kidney function tests, complete blood count, and lipogram (total cholesterol, triglycerides, and high density and low density lipoprotein cholesterol). No previous treatment was withdrawn.
No weaning period from treatment was necessitated. They were followed up weekly for 1-8 weeks (mostly 4 weeks).
FBS and 2 h PP were tested every week. The tablet of MC contained 20 mg of the polypeptide from the dried juice of the whole fruit. The dose given was four to six tablets/day, half an hour before meals (t.d.s.).
The pretreatment levels of FBS and 2 h PP for each patient was taken as his or her own control. The post-test treatment levels of FBS and 2 h PP for patients was taken as a mean of their weekly FBS and 2 h PP readings.
As this experiment is a case study, each one was discussed with normal standards.
| Results|| |
The results are summarized in [Table 1] and [Table 2].
The patients' age ranged from 25-70 years, but most of them were 40 years or older. Seven of the 18 patients were not on any hypoglycemic treatment; three patients were on insulin and the rest were on conventional oral hypoglycemic. Eight of 18 patients (44.4%) (four ♀ and four ♂) showed improvement in their FBS (range 17.2-61.2) and 2 h PP glucose levels (range 31.2-65.3%) (cases no. 1, 2, 3, 7, 8, 12, 13, 17). Three of 18 patients (two ♂ and one ♀) showed mild improvement (cases no. 9, 10, 11). Two of 18 patients (one ♀ and one ♂) showed improvement in PP blood sugar only (cases no. 4, 14). Four of 18 patients (three ♀ and one ♂) showed no improvement or deterioration in significance (cases no. 4, 5, 16, 18). Three patients had hyperlipidemia, two had elevated liver functions tests, and two were traced for only 1 week. One of 18 patients (a ♀) showed no improvement at 3 weeks, but showed improvement at 6 weeks (case no. 15).
| Discussion|| |
To date, close to 100 in-vivo studies have demonstrated the blood sugar lowering effect of bitter gourd fruit (MC). The fruit has also been shown to have the ability to enhance cell uptake of glucose to promote insulin release and to potentiate the effect of insulin.
MC has been ethnomedically used worldwide as a hypoglycemic - in Brazil, China, Cuba, India, Mexico, Nicaragua, Panama, Peru, etc. .
Substantial work has been carried out and documented on the positive hypoglycemic activity of MC on normal and diabetic laboratory animals ,,,,,,,. However, relatively few reports are available on its activity in humans.
This clinical trial dealt with 18 cases of type 2 diabetes mellitus. It was based on the long-lived, consistent, and updated role of MC as a hypoglycemic in experimental animals (rats and mice) and in individuals with type 2 diabetes mellitus. A study in 1982  documented eight patients with uncomplicated diabetes mellitus, who were given 50 mg/kg body weight of MC powder in milk, twice per day. They were on diet but not on any other treatment. They showed significant hypoglycemic effect, with no side effects at 1/2, 1, 1.5, and 2 h after glucose tolerance test.
The present study used 20 mg tablet from formulated dried juice of the whole fruit given at four to six tablets/day, half an hour before meals, t.d.s.
In 1986, Welihinda et al.  reported an improved glucose tolerance of 73% in a patient on MC. Kirti et al.  described early studies (1950-1974) in which karela's antidiabetic activity was observed. In a comprehensive review in King college, London, it was concluded that limited studies on humans have shown that karela fruit juice reduces fasting blood glucose tolerance on acute administration and that prolonged administration causes a lowering of glycosylated hemoglobin and decreases glucosuria and basal glycemia. This effect was also noticed in the present study, which recommends a treatment of 4 weeks or greater with MC .
An insulin secretagogue insulin mimetic activity of the fruit has been shown in vitro in animal experiments but not in vivo.
The review also provided data on a polypeptide insulin from the fruit that produces hypoglycemic effects in humans and animals on subcutaneous injection through the oral activity, which is questionable. Similarly, liver and reproductive system side effects recorded in animals have not been reported in humans despite the widespread use of the fruit medicinally.
In a study conducted in India, a significant fall (54%) in blood sugar was observed after treatment of rats for 3 weeks with an aqueous extract of the fruit, whereas the dried fruit showed a nonsignificant 25% fall .
In humans, the hypoglycemic effect was found to be highly significant (P < 0.01) at the end of the trial, but it was cumulative and gradual unlike that produced by insulin. The authors also believe in the adaptogenic properties of the fruit, indicated by the delay in appearance of the cataract, the secondary complication of diabetes mellitus, and relief of the neurological symptoms even before hypoglycemia occurs .
The present study used the dried juice and not the aqueous extract or dried fruit and recommends its use for 4 weeks or more for satisfactory results.
An analysis made in Bangladesh recorded a significant reduction (P < 0.001) in both FBS and PP serum glucose levels in 86% of cases, whereas 5% of cases showed lowering of FBS only. The present analysis revealed a decrease in FBS and 2 h PP in 44% of cases, mild improvement in 16% of cases, and decrease in PP only in 11% of cases (two cases) .
A study on the hypoglycemic action of MC among type 2 diabetic patients was conducted by Rosales and Fernando . They successfully tested the hypoglycemic effect of MC.
It was believed that the active ingredients in MC given orally to rats has neither insulin-like nor insulin-releasing activity, but they act by suppressing the transfer of glucose from the stomach to the small intestine and by inhibiting glucose transport to the brush border of the latter .
In Japan, the aqueous extract of MC was investigated, and a decrease in the blood glucose levels of mice 3 weeks after oral administration, and significantly lowered serum insulin were documented. They suggest that the antidiabetic effect of MC is derived at least in part, from a decrease in insulin resistance because of the noticed significant increase in GLUT4 protein in the plasma membrane of muscles. The present work did not assess insulin levels in the patients studied .
A research by Virdi et al.  in India concluded that the aqueous extract of powdered, fresh, unripe whole fruit of MC (as used in the present study) appears to be a safe alternative to reduce blood sugar, with no nephrotoxicity or hepatotoxicity by histological, biochemical parameters.
Basch et al.  reviewed the critical trials on different properties of MC, including hypoglycemic effects. They concluded that data are not sufficient to recommend its use in the absence of careful supervision and that monitoring of side effects and drug interactions, such as hypoglycemic coma, dropped fertility, and elevated liver enzyme, are necessary; this fact has been denied by the experiments of Raman and Lau .
The present trial agrees that elevated liver enzymes may interfere with the action of MC if not taken care of and properly controlled.
A review in India on the potential use of MC noted that there are only few reports on its clinical use in diabetes mellitus that have shown promising results .
Yibchok Anun et al.  documented in experimental STZ-induced diabetic rats, a slow-acting protein extract from the fruit pulp of MC, with insulin secretagogue, insulin mimetic activities to decrease blood glucose in vivo and enhance glucose uptake in lymphocytes and adipocytes.
In the Institute of Nutritional Science in Germany, it was reported that the hypoglycemic, metabolic effect of bitter gourd in cell culture, animal, and human studies were reported, although the mechanism of action is still under debate .
In a recent review conducted in Korea , MC has been reported to be beneficial in the treatment of type 2 diabetes [Figure 1] and [Figure 2].
|Figure 1: Momerdica charantia can be used as an effective oral adjunct hypoglycemic with no reportable clinical side effects. Treatment must continue for 4 weeks.|
Click here to view
| Conclusion|| |
On the basis of this individualized trial, it can be preliminarily concluded that MC can be used as an effective oral adjunct hypoglycemic with no reportable clinical side effects. It is advised to continue treatments for more than 4 weeks for possible tapering of the conventional hypoglycemics. In one case included in the experiments, it has been possible to stop insulin therapy in 7 weeks time. It is also concluded that hyperlipidemia and impaired liver function tests have to be carefully monitored and controlled before or during MC treatment.
| Acknowledgements|| |
The authors wish to express their thanks to the National Research Centre for the facilities offered to them through the project this paper resulted from it.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Bailey CJ, Day C. Traditional plant medicines as treatments for diabetes. Diabetes Care 1989; 12:553-564.
WHO. 1994 Expert Committee Diabetes Mellitus second report, Geneva, World Health Organization.
Bailey CJ, Day C, Turner SL, Leatherdale BA. Cerasee, a traditional treatment for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetes Res 1985; 2:81-84.
Duke JA. Hand book of medicinal herbs
. Boca Raton, FL: CRC; 1985.
Olaniyi AA. A neutral constituents of Momordica foetida
. Lloydia 1975; 38:361-362.
Vad BG. Place of Momordica charantia
in the treatment of diabetes mellitus. Maharashtra Med J 1960; 6:733-745.
Leatherdale BA, Panesar RK, Singh G, Atkins TW, Bailey CJ, Bignell AH. Improvement in glucose tolerance due to Momordica charantia
(karela). Br Med J (Clin Res Ed) 1981; 282:1823-1824.
Akhtar MS, Athar MA, Yacoub M. Effect of Momordica Charantia
on blood glucose levels of normal and alloxan diabetic rabbits. Planta Medica 1981; 42:205-212.
Sharma VN, Sogani RK, Arora RB. Some observations on the hypoglycaemic activity of Momordica charantia
. Indian J Med Res 1960; 48:471-477.
Welihinda J, Arvidson G, Gylfe E, Hellman B, Karlsson E. The insulin-releasing activity of the tropical plant momordica charantia. Acta Biol Med Ger 1982; 41:1229-1240.
Srivastava Y, Venkatakrishna-Bhatt H, Verma YH, Venkaiah K. Antidiabetic and adaptogenic properties of Momodica charantic
extract: an experimental and clinical evaluation. Phytother Res 1993; 7:285-289.
Raman A, Lau C. Anti-diabetic properties and phytochemistry of Momordica charantia
L. (Cucurbitaceae). Phytomedicine 1996; 2:349-362.
Taylor L. Healing power of rainforest herbs. A Guide to Understanding and Using Herbal Medicinals. Square One Oublisher, Inc., 2005.
Matsuda H, Li Y, Murakami T, Matsumura N, Yamahara J, Yoshikawa M. Antidiabetic principles of natural medicines. III. Structure-related inhibitory activity and action mode of oleanolic acid glycosides on hypoglycemic activity. Chem Pharm Bull (Tokyo) 1998; 46:1399-1403.
Sitasawad SL, Shewade Y, Bhonde R. Role of bittergourd fruit juice in STZ-induced diabetic state in vivo
and in vitro
. J Ethnopharmacol 2000; 73:71-79.
Miura T, Itoh C, Iwamoto N, Kato M, Kawai M, Park SR, Suzuki I. Hypoglycemic activity of the fruit of the Momordica charantia
in type 2 diabetic mice. J Nutr Sci Vitaminol (Tokyo) 2001; 47:340-344.
Virdi J Sivakami S, Shahani S, Suthar AC, Banavalikar MM, Biyani MK. Antihyperglycemic effects of three extracts from Momordica charantia
. J Ethnopharmacol 2003; 88:107-111.
Chaturvedi P. Role of Momordica charantia
in maintaining the normal levels of lipids and glucose in diabetic rats fed a high-fat and low-carbohydrate diet. Br J Biomed Sci 2005; 62:124-126.
Sathishsekar D, Subramanian S. Beneficial effects of Momordica charantia
seeds in the treatment of STZ-induced diabetes in experimental rats. Biol Pharm Bull 2005; 28:978-983.
Yibchok Anun S, Adisakwattana S, Yao SY, Sangvanich P, Roengsumran S, Hsu WH. Slow acting protein extract from fruit pulp of Momordica charantia
with insuling secretagogue and insulin mimetic activities. Biol Pharm Bull 2006; 29:1126-1131.
Akhtar, MS. Trial of Momrodica charantia
L. Powder in patients with maturity-onset diabetes. J Pakistan Med Assoc 1982; 32:106-107.
Welihinda J, Karunanayake EH, Sheriff MH, Jayasinghe KS. Effect of Momordica charantia
on the glucose tolerance in maturity onset diabetes. J Ethnopharmacol 1986; 17:277-282.
Kirti S, Kumar V, Nigam P, Srivatava P. Effect of Momordica charantia
Karela extract on blood and urine sugar in diabetes mellitus, study from a diabetic clinic. Clinician 1982; 46:26-29.
Ahmad N, Hassan MR, Halder H, Bennoor KS. Effect of Momordica charantia
Karela extract on fasting and post prandial serum glucose levels in NIDDM patients. Bangladesh Med Res Counc Bull 1999; 25:11-13.
Rosales R, Fernando R. An inquiry to the hypoglycemic action of Momordica charantia
among type II diabetic patients. Philippine J Inter Med 2001; 39:213-216.
Basch E, Gabardi S, Ulbricht C. Bitter melon (Momordica charantia
): a review of efficacy and safety. Am J Health Syst Pharm 2003; 60:356-359.
Grover JK, Yadav SP. Pharmacological actions and potential uses of Momordica charantia
: a review. J Ethnopharmacol 2004; 93:123-132.
Krawinkel MB, Keding GB. Bitter gourd (Momordica charantia
): a dietary approach to hyperglycemia. Nutr Rev 2006; 64(Pt 1):331-337.
Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK. Antidiabetic agents from medicinal plants. Curr Med Chem 2006; 13:1203-1218.
[Figure 1], [Figure 2]
[Table 1], [Table 2]